This study makes an assessment on the role of cats as potential carriers of pathogenic microorganisms. Totally, 360 samples from cats were collected and the pathogenic bacteria E. coli spp., Klebsiella spp., Enterobacter spp., Pseudomonas aeruginosa and Staphylococcus aureus were isolated from the different parts of cat’s body. All the isolated bacteria were confirmed through different biochemical and sugar fermentation tests. A total of 8 antibiotics were used against each isolated bacteria to check the resistivity pattern. E. coli spp. were found highly resistant against Amoxicillin, Erythromycin, Colistin sulphate, Vancomycin and Rifampicin antibiotics. Enterobacter spp. showed resistance against Amoxicillin, Colistin sulphate, Vancomycin and Rifampicin. The antibiotic resistivity pattern was observed in Klebsiella spp. against Amoxicillin, Erythromycin, Colistin sulphate, Vancomycin and Rifampicin antibiotics. Pseudomonas aeruginosa were found highly resistant against most of the antibiotics, which includes Amoxicillin, Erythromycin, Vancomycin, Meropenem, Ceftazidime, Colistin sulphate and Rifampicin antibiotics. Staphylococcus aureus species were observed as highly resistant to almost all antibiotics such as Oxytetracycline, Amoxicillin, Ceftazidime, Erythromycin, Meropenem, Colistin sulphate and Rifampicin. The Oxytetracycline antibiotic was recorded as most effective drugs against all isolates except Staphylococcus aureus which was observed susceptible to only one antibiotics that is Vancomycin. Whereas Meropenem and Ceftazidime antibiotics were found effective against Klebsiella spp. and Enterobacter spp. The current finding of this study suggests that cats can act as potential vector and reservoir of some important bacterial pathogens.