Mosquito pose a serious health concern for they are potential vectors of some of the most important diseases to human and animal, including, malaria, filariasis, West Nile virus and Rift Valley fever virus. A better understanding of the occurrence, abundance and distribution of mosquito species is often an important component of vector control and diseases control. This study was conducted at Souq Al- jum’aa municipality east of Tripoli district / Libya, during the period June to December 2016, 12 collection sites were selected randomly. Mosquito collections were carried out by CDC Miniature Black Light (UV) and breeding containers; to determine species composition at Souq Al- jum’aa municipality eastern Tripoli, Libya. Seven species representing three genera Aedes, Culex, Culiseta were Aedes detritus, Aedes caspius, Aedes dorsalis, Culex perexiguus, Culex pipiens, Culex laticinctus and Culiseta longiareolate. The dominant species was Culex pipiens with more than 84%. The presence of Aedes, Culex, Culiseta suggests the potential risk for vector-borne diseases. In this study, the results on Mosquito species may help with future planning of vector control measures. However, additional studies are recommended, to have an up-to-date list of the species present in the country.