The diversity of lady beetle fauna belonging to the subfamily Coccinellinae from Nepal Agricultural Research Council, Khumaltar, and Lalitpur is studied in this paper. In total, 14 species from 9 genera (Calvia, Cheilomenes, Coccinella, Coelophora, Harmonia, Hippodamia, Illeis, Oenopia and Propylea) and 1 tribe (Coccinellini) were collected and identified. The identified species were Calvia quatuordecimguttata (Linnaeus, 1758), Cheilomenes sexmaculata (Fabricius, 1781), Coccinella septempunctata Linnaeus, 1758, Coccinella transversalis Fabricius, 1781, Coelophora biplagiata (Swartz in Schönherr, 1808), Coelophora bissellata Mulsant, 1850, Harmonia sedecimnotata (Fabricius, 1801), Hippodamia variegata (Goeze, 1777), Illeis confusa Timberlake, 1943, Oenopia kirbyi Mulsant, 1850, Oenopia mimica Weise, 1902, Oenopia quadripunctata Kapur, 1963, Propylea dissecta (Mulsant, 1850) and Propylea luteopustulata (Mulsant, 1850).