The Larvicide Temephos has been widely used since the 1980’s to control the larvae of Aedes aegypti which is the main vector for Dengue Fever. The continuous usage of Temephos for a long period of time may cause resistance for mosquito larvae. The aim of this study was to monitoring the susceptibility status of the Ae. aegypti towards Temephos in five locations in Padang, West Sumatera, Indonesia. The Susceptibility Test was based by WHO’s Bioassay method (temephos 0.0120 mg/L) towards the Ae. aegypti larvae. The Ae. aegypti mosquito was resistant at Kp. Koto and Gn. Pangilun populations, tolerant at Cp. Tangah population, and still susceptible at KDP. Karakah and Ka. Dalam populations. In general, the concentration proposed as the diagnostic concentration by WHO was no longer effective in controlling the larvae of Ae. aegypti in Padang. The concentration of Temephos needed to control the larvae of Ae. aegypti in Padang was1-3.3 times higher than WHO’s diagnostic concentration (0.012 mg/L).