Both A. rufispinum and S. esakii possess 2n=10A+XY. Single chiasma per bivalent is the predominant feature in both the species. In A. rufispinum, all autosomal bivalents show thick terminal C-bands while in S. esakii, thin terminal bands are observed in four bivalents while one is C- negative. Y is C- positive and X is negative in both the species. All C-positive regions are DAPI/CMA3 bright. In A. rufispinum, each autosomal bivalent shows a specific distribution pattern of NORs while it is similar in all the bivalents in S. esakii. Linear characterization shows species specific distribution of C-bands, AT-GC rich DNA and NORs.