Boswellia Serrata Plant has found at Panchmari in central India. The methanolic extract of root of Boswellia Serrata has shown important broad spectrum antibacterial activity. This work is being carried out to separate and recognized the active composition of the plant for antibacterial. The main aim of our research of our methanolic extract of the plant was significantly reported for Phytochemical using standard procedures which release the presence of different kind of interaction with chemical existence.