Phylogenetic studies were performed on family Calliphoridae to deduce interspecific and intraspecific variations. Alignment of a total of 53 sequences comprising of 10 Calliphorid species was carried out. Also for population studies, 23 sequences were picked from the Gen Bank. Sarcophaga subvicina was chosen as the out group which got clearly separated in the phylogenetic tree. Interspecific variation among different species showed a wide range of 1-15%. Considerably high intraspecific divergences were observed when these sequences were compared with the global Gen Bank submissions. The intraspecific divergence observed for Chrysomya nigripes was 4%; for Lucilia sericata 5%; for Hemipyrellia ligurriens 1.7%; for Hemipyrellia pulchra 1.5%; for Chrysomya pinguis 2% and for Chrysomya albiceps it was 3%. While, for other species divergence was found to be within limits. These observations were also in agreement with previous research.