Wolbachia is an endosymbiontic alpha-proteobacteria found in insects. These bacteria are maternally transmitted reproductive manipulators affect the host biology of insects. The reproductive manipulations include cytoplasmic incomparability, feminization, parthenogenesis, male killing. Recent, meta analysis has estimated 65% of insects harbor Wolbachia. Significant recent research of using Wolbachia, focuses potential in biological control of insect pests and disease vectors. In the present study, Wolbachia and phageWO infection in agriculturally important insects was diagnosed and we discussed the its feature application prospects in biological control of agricultural pests and disease vectors.