The expression of regucalcin gene in cold acclimated diapause eggs of Dysdercus cingulatus was studied. The DNA and RNA were isolated from diapause induced eggs to low temperature and were used for the amplification of regucalcin gene. The PCR gel electrophoresis image shows that RNA and DNA obtained from the eggs had no traces of regucalcin gene. Thus present study shows that regucalcin gene was not expressed in diapause eggs and it may not play any role in cold tolerance diapause eggs.