Mosquitoes are vectors of various diseases since time immemorial. Madhya Pradesh is endemic to various mosquito borne diseases like malaria, dengue and chikungunya. A survey on species composition and seasonal variation of mosquitoes found in Gwalior was conducted in 2014. A complete year study was conducted to determine the abundance & diversity of various mosquito species present in both rural and urban areas of Gwalior district. Various breeding habitats for immature forms of mosquitoes were also studied during this tenure, as larval residing places plays an important role in disease transmission. A total number of ---- mosquitoes were collected that belonged to 4 genera and 9 species. The most abundant species in rural areas was found to be Culex quinquefasciatus followed by Aedes albopictus while in urban areas Culex quinquefasciatus was found to be most dominating species followed by Aedes aegypti species. The various indices like Margalef’s index of richness (D_mg), Shannon-weaver index of Diversity (H’), Simpson index of dominance were also calculated for mosquito richness, diversity and dominance in rural and urban areas of Gwalior respectively. Even the relative frequency and density were analysed for the study area during study tenure. The present study will be essential in controlling vector borne diseases and implementing vector control strategies against the mosquito species of Gwalior district of Madhya Pradesh, India.