Comparative study of aedeagus is made in one hundred and two species of grasshoppers representing fifty-nine genera belonging to the family Acrididae. Its taxonomic significance is shown. Divided, undivided or flexured conditions of aedeagus is taken as familial character. Apical valve of aedeagus longer or shorter than basal valve is considered as generic character. Shape of apical and basal valves is suggested as specific character.