International Journal of Entomology Research

Authors
AR Gaikwad, GP Bhawane

Abstract
Liatongus rhadamistus is common dung beetle found in South-Western Maharashtra, India. Characteristics of some Carbohydrases were studied from mid gut of male and female of this dung beetle. Most of the Carbohydrases showed their maximum activities at pH 7.2.While some Carbohydrase were showing pH optima at 5.6 (Cellulase) and 6.0 (Trehalase) in both sexes. Temperature optima for all Carbohydrases which were studied in both sexes occurred at 45⁰C. The Km value of Amylase in male was 1.143% and in female was 1.60%.The Km values of Invertase enzyme were 5.56 x 10^-3 M in male and 1.297 ^-2 M in female beetles. The 50% denaturation of Amylase activity was occurred within 46 minutes in male and 45 minutes in female at 60⁰ C. At same temperature the half-life of Invertase was 21 minutes in male and 24 minutes in female. The digestion period of 60 minutes were fitted very well within the linear part of enzymatic action for both enzyme and sex. All Carbohydrases which were studied in this dung beetle shows that Amylase contribute significantly in digestion of carbohydrate in both sexes.