Quantitative assessment of royal jelly collected at fifferent time interval in honey bees Apis mellifera
Khalida Hamid Abbasi, Muhammad Shafiq, Khawer Jawad Ahmad, Asif Razzaq, Muhammad Arshad Ullah
The trial has been conducted at research farm of Bee Keeping and Hill Fruit Pest Research Station Rawalpindi from mid-February to April with optimum temperature 20-30 0C during the period of 2012-2014. Three treatment time intervals 48, 72 and 96 hrs were taken for study purpose. Twenty (20) artificially made queen cups from beeswax with a special cell forming dye were selected and fitted in a wooden hive frame. In each queen cup a twenty four hours old young larvae of worker bee from a best performing colony was grafted with the help of grafting needle for the purpose of royal jelly extraction. The results showed that extraction of royal jelly has significant (P>0.05) results in treatment having 72 hrs time interval over other treatments 48 hrs and treatment 96 hrs time intervals respectively. Analysis of variance indicated significantly different effects for different treatments (F>215.09, P>F=0.0005). Least square distance (LSD) for each treatment showed that all treatments had significantly different effects from each other. This protocol concluded that the queen cups took time interval of 72 hrs extracted maximum royal jelly after larval graft results high production proved that this time interval provide optimum condition for royal jelly production, while increasing the time to 96 hrs reduced the royal jelly extraction.