Isolation and biochemical identification of Mid Gut microbiota of wild caught and lab reared Anopheles Stephensi and their possible role in disease transmission
Ankita Kumari, Arti Prasad
For description the midgut microbiota diversity to find the seeker bacterial for the transmission of malaria, the microbiota of wild adult Anopheles stephensi mosquito and adult lab-reared Anopheles stephensi mosquito midgut which were collected from southern udaipur was studied using a culture dependent, biochemical-based technique. Gram staining and specific media were used for the identification of Gram-positive and Gram–negative bacterial colonies. Isolated and selected colonies were identified by different physiological, biochemical tests and techniques. Eighteen species in 9 genera in which six Gram-negative: Aeromonas, Myroids, Pseudomonas, Klebsiella, Enterobacter and Shewanella and three Gram-positive Microbacterium, Enterococcus and Rhodococcus bacterial colonies were isolate and identified in the mid gut of the wild cough anopheles stephensi mosquito. 12 Gram-negative species in three genera including Pseudomonas, Myroides and Aeromonas were isolated and identified from lab-reared anopheles stephensi mosquitoes. Pseudomonas and Aeromonas with a frequency of 51% and 14% field caught and in lab reared midgut with a frequency of 54% and 20% were the most common midgut symbionts. Three genera have been isolated from both wild and lab reared mosquitoes were Pseudomonas, Aeromonas and Myroides. Fastidious growth of bacteria shown in inexpensive media, which were Gram negative, and found dominant in both wild and lab reared anopheles stephensi mosquitoes and presence in other malaria vectors.
Ankita Kumari, Arti Prasad. Isolation and biochemical identification of Mid Gut microbiota of wild caught and lab reared Anopheles Stephensi and their possible role in disease transmission. International Journal of Entomology Research, Volume 7, Issue 4, 2022, Pages 18-23