International Journal of Entomology Research

International Journal of Entomology Research


International Journal of Entomology Research
International Journal of Entomology Research
Vol. 6, Issue 3 (2021)

Molecular cloning and confirmation of MSH2, an essential gene of human, involved in DNA mismatch repair


Sarita Kumari, C Rajesh

Mismatch repair (MMR) is one such pathway that repair mismatches generated during DNA replication. Errors in MMR cause certain types of cancer including hereditary non-polyposis colorectal cancer, genome instability, abnormalities in meiosis, sterility in mammalian systems and resistance to certain chemotherapeutic agents. In eukaryotes, MSH2 (MutS homologue 2) is a major player in MMR, acting in combination with MSH3 or MSH6 as a heterodimer. MSH2 has been involved in a variety of processes which protect the integrity of the genome. In this study hMSH2 gene was cloned to identifying their role and understanding the functional diversity of protein that will contribute towards the repair mechanisms. Using in frame designed primers through PCR strategy and cloned full-length cDNA fragment hMSH2 gene into pET32b expression vector. The sequence contained an open reading frame of 2805 bp coding for a putative protein of 935 amino acids. Recombinant C-3 clone (pET32b-hMSH2) observed as positive after restriction digestion with expected band size (~8.6kb). This recombinant clone of hMSH2 gene, further used to express the protein and purified protein used to understand the role of mismatch repair protein MSH2.
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How to cite this article:
Sarita Kumari, C Rajesh. Molecular cloning and confirmation of MSH2, an essential gene of human, involved in DNA mismatch repair. International Journal of Entomology Research, Volume 6, Issue 3, 2021, Pages 47-53
International Journal of Entomology Research International Journal of Entomology Research