Quantitative assessment of enzymes is reliable to understand the stress imposed on the organism by environmental pollutants. In the present investigation, the effect of sublethal concentration of nickel chloride on acid and alkaline phosphatases in liver and kidney of the fish Labeo rohita was studied The result showed in general, a significant (P< 0.05) reduction in the activity of both the enzymes in liver and kidney, but more pronounced in kidney than liver. The alteration in enzymatic changes indicated that nickel chloride is very hazardous to the fish.