International Journal of Entomology Research

Authors
Dina V Rombot, Jantje Pelealu, Max Tulung, Jantje Memah, Mokosuli Yermia Semuel

Abstract
The position of species of cat flea is still a lot of debate. Cat flea are one of the insect species that succeeded in converging evolution, among others, caused by human-assisted migration. Identification using morphological data is not enough to determine the position of species of cat flea. Research has been conducted to find out the position of species of cat flea from the city of Manado. The stages of the research are cat flea sampling, DNA extraction and purification, amplification and visualization of the CO1 gene, sequencing and sequence analysis. The results of this research showed based on sub unit 1 (CO1) cytochrome oxidase gene, cat flea isolated from cats in Manado city have a similarity of 99% with the CO1 gene sequence Ctenochepalides felis felis [KP687811.11]. However, the phylogeny tree formed shows younger Ctenochepalides felis from Manado based on evolutionary time. The molecular barcoding profile of the C. felis CO1 gene cannot be constructed on the BOLD system (www.boldsystems.org).