The effect of road networks, traffic on roads affecting the biodiversity. Increased road networks in India fragmenting the natural habitat of animals. The affect of road networks on mortality of animal studies are very fragmentary and most of them are focused on Amphibians, Reptiles, and small mammals. To keep an ecosystem healthy invertebrates are key members and participate greatly in the healthy ecosystem maintenance. There are no such studies conducted from Kalaburagi region of Karnataka; hence the present survey was conducted. A total of 180 individuals were recorded during the study period, belongs to 4 families, 20 genera and 35 species. Among 4 families Nymphalids were highly prone to getting killed on the roads and least killed were Lycaenids. Proper management of road verges serves as a good habitat for butterflies.