The preferences on the oviposition and pupation sites are considerably important for maximizing the survival of offspring and reproduction of the butterflies, In this present study, we aimed to investigate the preference of H. bolina oviposition site on its host plant namely Laportea interupta (Urticaceae). Our study found that the majority of H. bolina butterflies preferred to lay the eggs on the underside of leaves(73%) than the upper side, petiole, oviposition mistake, and stems.The young leaves were preferable than the mature leaves, petiole, seedling, and stems. The most frequent type of oviposition of H. bolina was in a cluster. Based on the substrate, H. bolina pupae were commonly observed on the non-hostplant substrate particularly on the living stems. The siteswith the height ranged from 31-60 cm above the ground surface were preferable as the place for the pupation. The information about the preferences on the oviposition and pupation sites could be useful for further conservation policies of the butterflies both in situ and ex-situ.