Vol. 3, Issue 4 (2018)
Identification and Characterization of gut associated bacteria in the larvae of Spodoptera litura using 16s rRNA gene
Author(s): Nimna P, Kavipriya J, Sankaraiyah K
Abstract: The aim of the present study was to identify and characterize the gut associated bacteria in Spodoptera litura. The bacterial flora of S. litura was determined by using both culture dependent and culture independent. In total, 31 bacteria were characterized based on their morphological, biochemical and molecular characteristics. Out of thirty one, six isolates were gram-negative bacteria, twenty seven isolates were catalase producers, nine isolates were lipase producers, twenty isolates were amylase producers and twenty isolates were gelatinase producers. The bacteria isolated from S. litura were determined as Pseudomonas aeruginosa (SLI03) and Bacillus tequilensis (SLI23) by using 16S rRNA gene partial sequencing. The sequences were submitted in NCBI GenBank and given accession numbers KY002523 and KY002524.