Expression patterns of epsilon glutathione S – transferases genes in developmental stages of susceptible and DDT resistant lines of Anopheles arabiensis strains
Yayo AM, Ado A, Habibu UA, Mohammed BR, Ebere N, Hemingway J
Expression patterns of three epsilon GST genes were studied in developmental stages of two strains of Anophelesarabiensis (An. arabiensis)which differed in susceptibility to DDT. The mosquito strains reared to several generations were successively exposed to and selected for DDT resistance. Quantitative real-time PCR was used to compare the transcript levels of these genes in ontogenic stages of the mosquitoes. The expression patterns of GSTe1 and GSTe2 varied relatively in proportion to resistance status and sequential life stages while GSTe4 was expressed invariably. Further investigation is needed to understand the factors regulating the expression of the individual genes in each life stage of An. arabiensis.