International Journal of Entomology Research

International Journal of Entomology Research


ISSN: 2455-4758

Vol. 3, Issue 2 (2018)

Storage protein in the haemolymph of 6th instar larvae of Spodoptera mauritia Boisd. (Lepidoptera: Noctuidae) is increased by the ecdysone mimic, methoxyfenozide

Author(s): Linvy Vincent, Sridhu Prakash, Reshma RM, Resmitha C, Kannan Vadakkadath Meethal
Abstract: Spodoptera mauritia is a sporadic pest of paddy which causes, great economic loss. Insect Growth Regulators (IGRs) belong to a group of compounds which interfere with normal growth, development and reproduction in insects by disrupting hormonally regulated physiological processes. As IGRs are more target-specific, non-persistent, biodegradable and environmentally benign substances with less toxicity to non-target organisms, they are ideal for pest control programs. In this study we demonstrate the effect of methoxyfenozide, an ecdysone mimic, on the haemolymph protein profile of 6th instar larvae of Spodoptera mauritia. When the sixth instar day 0 larvae of Spodoptera mauritia was treated with different concentrations of methoxyfenozide, there was a statistically significant increase in the haemolymph protein concentration after 24 hours. The haemolymph collected after 24 hours when subjected to SDS-PAGE, there was an increase in the intensity of the major protein band of 83kDa compared to control. Storage proteins are the major proteins in the haemolymph of last instar lepidopteran larvae. Whether the increase in storage protein concentration by methoxyfenozide is related to insecticide detoxification and resistance is worth investigating and studies in this direction are ongoing.
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