Acanthosomatidae is a small family of pests and decomposers, comprising around 230 species worldwide. Cytogenetic data for Indian Acanthosomatidae is available for only six species. In the present paper, cytogenetic details of two Acanthosomatid species, Acanthosoma rufispinum
and Sastragala esakii
collected from Himachal Pradesh have been described for the first time.
Both A. rufispinum and S. esakii possess 2n=10A+XY. Single chiasma per bivalent is the predominant feature in both the species. In A. rufispinum, all autosomal bivalents show thick terminal C-bands while in S. esakii, thin terminal bands are observed in four bivalents while one is C- negative. Y is C- positive and X is negative in both the species. All C-positive regions are DAPI/CMA3 bright. In A. rufispinum, each autosomal bivalent shows a specific distribution pattern of NORs while it is similar in all the bivalents in S. esakii. Linear characterization shows species specific distribution of C-bands, AT-GC rich DNA and NORs.